The Thyroid Gland's Glycosylation: Aspect of Glycoprotein Function in Thyrocyte Physiology and Thyroid Disorders

Objective: The focus of this study is on the role glycoproteins in normal thyrocyte activity and pathologies thyroid. Study Design: Observational Place Duration: was carried at Pak International Medical College Hospital from November 2021 to January 2022 Methods: There were 38 patients both genders presented study. Detailed demographics enrolled cases included age, sex body mass index recorded after taking informed written consent. 19 had benign diffuse goiter group I (controls) include II. Using a nonspecific proteomic strategy, such as double difference (2D-DIGE) electrophoresis lattice laser desorption/ionization time-of-flight masses spectrometry, we able determine which proteins present thyroid tissue (MALDI-TOF-MS). Results: Among 92 significantly different between BDG control group, 47 found be elevated 45 lowered (1.5-fold change, ANOVA, p 0.05). Bioinformatics analysis Ingenuity Pathway Analysis has connected dysregulation ERK1/2, Glutathione peroxidase, NADPH oxidase signaling pathways damage, endocrine system dysfunction, cancer (IPA). Conclusion: Low levels thyroglobulin, dysregulated glycolysis, an uptick pro-oxidant peroxidase enzymes proteome with BDG. also finding cytoskeletal proteins, redox glycolytic pathways. Keywords: Thyroid Gland's, Benign Diffuse Goiter, Proteins, Glycosylation